Tracking drug action in living cells
  • CETSA® MS enables proteome-wide target identification without the need for a label on the compound or the protein
  • Thermal stability can be used to monitor protein-ligand interactions for up to 7,000 proteins in living cells or lysates
  • The technology provides an unbiased measure of drug-target engagement and facilitates identification of markers for drug efficacy and toxicity


Further reading

Savitski M.M., et al. (2014). Tracking cancer drugs in living cells by thermal profiling of the proteome. Science, 346(6205):1255784. [Link]

Berndt, J. D., & Wong, W. (2015). 2014: Signaling Breakthroughs of the Year. Sci Signal, 8(358), eg1. [Link]

Reinhard F.B., et al. (2015). Thermal proteome profiling monitors ligand interactions with cellular membrane proteins. Nat Methods. 12(12):1129-31. [Link]

Martinez Molina, D., Jafari, R., Ignatushchenko, M., Seki, T., Larsson, E.A., Dan, C., Sreekumar, L., Cao, Y., Nordlund, P. (2013). Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay. Science. 341(6141):84-7. [Link]

Proteome-wide CETSA® MS services are performed under a license from Pelago Bioscience AB.


Typical protein melting curves
Proteome-wide CETSA® (also know as Thermal Proteome Profiling) can be used to study protein-ligand interactions in a proteome-wide manner. Science Signaling elected the technology one of the Breakthroughs of the year 2014.